リポソーム
Kamiya Laboratory
Graduate School of Science and Technology Gunma University
Biomolecular Chemistry Laboratory

Papers   Review Papers    Conference

Papers

2021

  1. Hiroki Yasuga, Emre Iseri, Xi Wei, Kerem Kaya, Giacomo Di Dio, Toshihisa Osaki, Koki Kamiya, Polyxeni Nikolakopoulou, Sebastian Buchmann, Johan Sundin, Shervin Bagheri, Shoji Takeuchi, Anna Herland, Norihisa Miki and Wouter van der Wijngaart, “Fluid interfacial energy drives the emergence of three-dimensional periodic structures in micropillar scaffolds” Nature Physics, (2021). link

  2. Koki Kamiya*, Chika Arisaka, and Masato Suzuki, “Investigation of Fusion between Nanosized Lipid Vesicles and a Lipid Monolayer toward Formation of Giant Lipid Vesicles with Various Kinds of Biomolecules” Micromachines, Vol. 12 (2021) 133. link

  3. Tetsuya Yamada, Hirotaka Sugiura, Hisatoshi Mimura, Koki Kamiya, Toshihisa Osaki, and Shoji Takeuchi, “Highly sensitive VOC detectors using insect olfactory receptors reconstituted into lipid bilayers” Science Advances, Vol. 7 (2021) eabd2013. link

  4. Koki Kamiya, Toshihisa Osaki, and Shoji Takeuchi, “Formation of nano-sized lipid vesicles with asymmetric lipid components using a pulsed-jet flow method” Sensors and Actuators B: Chemical, Vol. 327 (2021) 128917. link

  5. Nobuo Misawa, Satoshi Fujii, Toshihisa Osaki, Koki Kamiya, Toshihisa Osaki, and Shoji Takeuchi, “Efficient Lipid Bilayer Formation by Dipping Lipid-Loaded Microperforated Sheet in Aqueous Solution” Micromachines, Vol. 12 (2021) 53. link

2020

  1. Yoshihisa Ito, Yusuke Izawa, Toshihisa Osaki, Koki Kamiya, Nobuo Misawa, Satoshi Fujii, Hisatoshi Mimura, Norihisa Miki, and Shoji Takeuchi, “A Droplet-Based Lipid-Bilayer Device for Pumpless Sample Exchange” Micromachines, Vol. 11 (2020) 1123. link

  2. Yoshihisa Ito, Toshihisa Osaki, Koki Kamiya, Tetsuya Yamada, Norihisa Miki, and Shoji Takeuchi, “Rapid and resilient detection of toxin pore formation using a lipid bilayer array”Small, Vol. 16 (2020) 2005550. link

  3. Saori Nonaka, Emil Salim, Koki Kamiya*, Aki Hori, Firzan Nainu, Rangga Meidianto Asri, Ayu Masyita, Takumi Nishiuchi, Shoji Takeuchi, Noriyuki Kodera*, and Takayuki Kuraishi*, “Molecular and functional analysis of pore-forming toxin Monalysin from entomopathogenic bacterium Pseudomonas entomophila” Frontiers in Immunology, Vol. 11 (2020) 520. link

2019

  1. Testuya Yamada, Koki Kamiya, Toshihisa Osaki, and Shoji Takeuchi “A pumpless solution exchange system for nanopore sensors” Biomicrofludics, Vol. 13 (2019) 064104. link

  2. Koki Kamiya, Toshihisa Osaki, and Shoji Takeuchi, “Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method” RSC Advances, Vol. 9 (2019) pp.30071-30075.  link

  3. Masahide Gotanda‡, Koki Kamiya‡, Toshihisa Osaki, Norihisa Miki, and Shoji Takeuchi, “Automatic generation system of cell-sized liposomes” Sensors and Actuators B: Chemical, Vol. 292 (2019) pp.57-63. ‡Equal contribution  link

  4. Nobuo Misawa, Satoshi Fujii, Koki Kamiya, Toshihisa Osaki, Tomoyuki Takaku, Yasuhiro Takahashi, and Shoji Takeuchi, “Construction of a Biohybrid Odorant Sensor Using Biological Olfactory Receptors Embedded into Bilayer Lipid Membrane on a Chip” ACS Sensors, Vol. 4 (2019) pp.711-716. link

2018

  1. Koki Kamiya, Toshihisa Osaki, Kenji Nakao, Ryuji Kawano, Satoshi Fujii, Nobuo Misawa, Masatoshi Hayakawa, and Shoji Takeuchi, “Electrophysiological measurement of ion channels on plasma/organelle membranes using an on-chip lipid bilayer system” Scientific Reports, Vol. 8 (2018) 17498. link

  2. Satoshi Fujii, Koki Kamiya, Toshihisa Osaki, Nobuo Misawa, Masatoshi Hayakawa, and Shoji Takeuchi, “Purification-Free MicroRNA Detection by Using Magnetically Immobilized Nanopores on Liposome Membrane” Analytical Chemistry, Vol. 90 (2018) pp. 10217-10222. link

  3. Hiroki Yasuga, Koki Kamiya, Shoji Takeuchi, and Norihisa Miki, “Self-generation of two-dimensional droplet array using oil-water immiscibility and replacement” Lab on a Chip, Vol. 18 (2018) pp.1130-1137. link

  4. Masahide Gotanda‡, Koki Kamiya‡, Toshihisa Osaki, Satoshi Fujii, Nobuo Misawa, Norihisa Miki, and Shoji Takeuchi, “Sequential generation of asymmetric lipid vesicles using a pulsed-jetting method in rotational wells” Sensors and Actuators B: Chemical, Vol. 261 (2018) pp.392-397. ‡Equal contribution link

  5. Koki Kamiya‡, Yuta Abe‡, Kosuke Inoue, Toshihisa Osaki, Ryuji Kawano, Norihisa Miki, and Shoji Takeuchi, “Well-controlled cell-trapping systems for investigating heterogeneous cell-cell interactions” Advanced Healthcare Materials, Vol.7 (2018) 1701208. ‡Equal contribution  (selected as the inside front cover) link

  6. Yusuke Izawa, Toshihisa Osaki, Koki Kamiya, Satoshi Fujii, Nobuo Misawa, Shoji Takeuchi, and Norihisa Miki, “Suppression of sloshing by utilizing surface energy and geometry in microliter cylindrical well” Sensors and Actuators B: Chemical, Vol. 258 (2018) pp.1036-1041. link

2017

  1. Hiroki Yasuga, Kosuke Inoue, Ryuji Kawano, Masahiro Takinoue, Toshihisa Osaki, Koki Kamiya, Norihisa Miki, and Shoji Takeuchi, “Serial DNA Relay in DNA logic Gates by electrical Fusion and Mechanical Splitting of Droplets” PloS ONE, vol. 12, (2017) e180876.link

  2. Satoshi Fujii, Aiko Nobukawa, Toshihisa Osaki, Yuya Morimoto, Koki Kamiya, Nobuo Misawa and Shoji Takeuchi, “Pesticide vapor sensing using an aptamer, nanopore, and agarose gel on a chip” Lab on a Chip, Vol. 17 (2017) pp.2421-2425. link

  3. Ryuji Kawano, Nao Horike, Yuh Hijikata, Mio Kondo, Arnau Carne-Sanchez, Partrick Larpent, Shuya Ikemura, Toshihisa Osaki, Koki Kamiya, Susumu Kitagawa, Shoji Takeuchi, and Shuhei Frukawa, “Metal-Organic Cuboctahedra for Synthetic Ion Channels with Multiple Conductance States” Chem, Vol. 2 (2017) pp.393-403. link

  4. Yuriko Oyama, Toshihisa Osaki, Koki Kamiya, Masatoshi Sawai, Masatoshi Sakai, and Shoji Takeuchi, “A sensitive point-of-care testing chip utilizing superabsorbent polymer for the early diagnosis of infectious disease” Sensors and Actuators B: Chemical, Vol. 240 (2017) pp.881-886. link

2016

  1. Koki Kamiya, Ryuji Kawano, Osaki Toshihisa, Kazunari Akiyoshi, and Shoji Takeuchi, “Cell-sized asymmetric lipid vesicles facilitate the investigation of asymmetric membranes” Nature Chemistry, Vol. 8 (2016) pp.881-889. link

  2. Tetsuhiko Teshima, Hiroaki Onoe, Soichiro Tottori, Hiroka Aonuma, Takeomi Mizutani, Koki Kamiya, Hirotaka Ishihara, Hirotaka Kanuka, and Shoji Takeuchi, “High-Resolution Vertical Observation of Intracellular Structure Using Magnetically Responsive Microplates” Small, Vol. 12 (2016) pp.366-3373. link

  3. Hiroki Yasuga, Ryuji Kawano, M Takinoue, Yutaro Tsuji, Toshihisa Osaki, Koki Kamiya, Norihisa Miki, and Shoji Takeuchi, “Logic Gate Operation by DNA Translocation through Biological Nanopores” PloS ONE, vol.11 (2016) e0149667. link

2015-2010

  1. Yuta Abe‡, Koki Kamiya‡, Toshihisa Osaki, Hirotaka Sasaki, Ryuji Kawano, Norihisa Miki and Shoji Takeuchi, “Nonlinear concentration gradients regulated by the width of channels for observation of half maximal inhibitory concentration (IC50) of transporter proteins” Analyst, Vol. 140 (2015) pp.5557-5562. ‡Equal contribution link

  2. Marie Tani, Ryuji Kawano, Koki Kamiya, and Ko Okumura, “Towards combinatorial mixing devices without any pumps by open-capillary channels: fundamentals and applications” SCIENTIFIC REPORTS, Vol. 5 (2015) 10263. All authors contributed equally to the work. link

  3. Ryuji Kawano, Yutaro Tsuji, Koki Kamiya, Taiga Kodama, Toshihisa Osaki, Norihisa Miki, and Shoji Takeuchi, “A Portable Lipid Bilayer System for Environmental Sensing with a Transmembrane Protein” PloS ONE, Vol. 9(7) (2014) : e102427 link

  4. Takaaki Mori, Koki Kamiya, Masahiro Tomita, Tetsuro Yoshimura, and Kanta Tsumoto, “Incorporation of adenylate cyclase into membranes of giant liposomes using membrane fusion with recombinant baculovirus-budded virus particles” Biotechnology Letters, Vol. 36 (2014) pp.1253-1261. link

  5. Tetsuhiko Teshima, Hiroaki Onoe, Hiroka Aonuma, Kaori Kuribayashi-Shigetomi, Koki Kamiya, Taishi Tonooka, Hirotaka Kanuka, and Shoji Takeuchi, “Magnetically Responsive Microflaps Reveal Cell Membrane Boundaries from Multiple Angles” Advanced Materials, Vol. 26 (2014) pp. 2850–2856. link

  6. Tetsuhiko Teshima, Hiroaki Onoe, Kaori Kuribayashi-Shigetomi, Hiroka Aonuma, Koki Kamiya, Hirotaka Ishihara, Hirotaka Kanuka, and Shoji Takeuchi, “Parylene Mobile Microplates Integrated with an Enzymatic Release for Handling of Single Adherent Cells” Small, Vol. 10 (2014) pp.912-921. link

  7. Yutaro Tsuji, Ryuji Kawano, Toshihisa Osaki, Koki Kamiya, Norihisa Miki and Shoji Takeuchi, “Droplet Split-and-Contact Method for High-Throughput Transmembrane Electrical Recording” Analytical Chemistry, Vol. 85 (2013) pp.10913-10919. link

  8. Ryuji Kawano, Yutaro Tsuji, Koji Sato, Toshihisa Osaki, Koki Kamiya, Minako Hirano, Toru Ide, Norihisa Miki and Shoji Takeuchi, “Automated Parallel Recordings of Topologically Identified Single Ion Channels” SCIENTIFIC REPORTS, Vol. 3 (2013) 1995. link

  9. Taiga Kodama, Toshihisa Osaki, Ryuji Kawano, Koki Kamiya, Norihisa Miki, Shoji Takeuchi, “Round-Tip Dielectrophoresis-Based Tweezers for Single Micro-Object Manipulation” Biosensors and Bioelectronics, Vol. 47 (2013) pp.206-212. link

  10. Yutaro Tsuji, Ryuji Kawano, Toshihisa Osaki, Koki Kamiya, Norihisa Miki and Shoji Takeuchi, “Droplet-based lipid bilayer system integrated with microfluidic channels for solution exchange” Lab on a Chip, Vol.13 (2013) pp.1476-1481. link

  11. Yuriko Oyama, Toshihisa Osaki, Koki Kamiya, Ryuji Kawano, Tsutomu Honjoh, Haruki Shibata, Toru Ide and Shoji Takeuchi, “A glass fiber sheet-based electroosmotic lateral flow immunoassay for point-of-care testing” Lab on a Chip, Vol. 12, (2012) pp.5155-5159.link

  12. Yusuke Kishi, Tetsuya Higuchi, Shirly Phoon, Yasuo Sakamaki, Koki Kamiya, Gabriela Riemekasten, Kazunari Akiyoshi, Martin Weigert, and Takeshi Tsubata, “Apoptotic marginal zone deletion of anti-Sm/RNP B cells” Proceedings of the National Academy of Sciences, vol. 109 no.20 (2012) pp. 7811-7816. link

  13. Hirotaka Sasaki, Ryuji Kawano, Toshihisa Osaki, Koki Kamiya, and Shoji Takeuchi, “Single-Vesicle Analysis of ATP-binding cassette transporters in microfluidic Channels” Lab on a Chip, Vol. 12, No.4, (2012) pp. 702-704. link

  14. Koki Kamiya, Kanta Tsumoto, Tetsuro Yoshimura, and Kazunari Akiyoshi, “Cadherin-integrated liposomes with potential application in a drug delivery system” Biomaterials, Vol. 32 (2011) pp.9899-9907. link

  15. Koki Kamiya, Kanta Tsumoto, Satoko Arakawa, Shigeomi Shimizu, Ikuo Morita, Tetsuro Yoshimura, and Kazunari Akiyoshi, “Preparation of connexin43-integrated giant liposomes by a baculovirus expression-liposome fusion method” Biotechnology and bioengineering, Vol. 107 (2010) pp. 836-843. (spotlightに選出) link

  16. Koki Kamiya, Jun Kobayashi, Tetsuro Yoshimura, and Kanta Tsumoto, “Confocal microscopic observation of fusion between baculovirus budded virus envelopes and single giant unilamellar vesicles” Biochimica et Biophysica Acta – Biomembranes, Vol. 1798 (2010) pp. 1625-1631. link


Review Papers

   

English

  1. Koki Kamiya, “Development of artificial cell models using microfluidic technology and synthetic biology” Micromachines, Vol. 11 (2020) 559. link

  2. Koki Kamiya and Shoji Takeuchi, “Giant liposome formation toward the synthesis of well-defined artificial cells,” Journal of Materials Chemistry B, Vol. 5 (2017) pp.5911-5923. link
   

Japanese

  1. 神谷厚輝 “リン脂質非対称膜リポソームの形成と機能観察” 化学と工業 (2019) Vol.72 No.12 pp.1049-1050.

  2. 神谷厚輝 “人工細胞膜の創成と機能観察” 化学とマイクロ・ナノシステム (2019) Vol.18 No.2 pp.16. link

  3. 神谷厚輝、竹内昌治“細胞サイズリポソームのエンジニアリング” 生物物理 (2018) Vol.58 No.6 pp.291-296.

  4. 神谷厚輝、竹内昌治“マイクロデバイスを利用した人工脂質二重膜によるタンパク質機能解析” 生体の科学 (2018) Vol.69 No.3 pp.252-257.

  5. 神谷厚輝、竹内昌治“リン脂質非対称膜組成の人工細胞膜作製と生体分子相互作用観察” 生化学 (2018) Vol.90, pp.225-229. link

  6. 藤井聡志、神谷厚輝、竹内昌治“人工細胞膜を利用した1分子検出” 現代化学 (2016) No.545, pp.53-57.

  7. 神谷厚輝、大崎寿久、竹内昌治“人工細胞膜作製とシングルイオンチャネル計測” Electrochemistry (2015) Vol.83, No.12, pp.1096-1100.

  8. 神谷厚輝、大崎寿久、竹内昌治 “液滴接触法による人工細胞膜上でのチャネルタンパク質機能と応用” 分析化学 (2015) Vol.64 No.6 pp.441-449.

  9. 神谷厚輝 “人工細胞モデル創成” 生体の科学 (2014) Vol.65 No.5 pp.492-493.